Cas-L (Nedd9, neural precursor cell expressed, developmentally down-regulated gene 9) gene knockout mice. A 1.2 kb region including exon 2 was replaced with EGFP and a floxed neomycin resistant cassette. Homozygous mutant mice show reduced numbers of lymphocytes in secondary lymphoid organs and an almost complete loss of marginal zone B (MZB) cells in the spleen. C（3〜6か月） 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Immunol., 175, 3492-3501 (2005). Heterozygote x Wild-type [C57BL/6JJcl] Cre/loxP system TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] Mineo KUROKAWA C (3-6 months) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 175, 3492-3501 (2005). 東京大学医学部 (血液 ・ 腫瘍内科) 瀬尾幸子先生が作成 (2001年), 分与 (2012年)。TT2 ES細胞を用いて作出。 Developed by Sachiko Seo, Faculty of Medicine, University of Tokyo in 2001. The targeting vector was transferred into TT2 ES cells derived from (C57BL/6 x CBA) F1. The mutant mice were backcrossed to C57BL/6. 黒川　峰夫 <a href='https://brc.riken.jp/mus/pcr05457'>Genotyping protocol -PCR-</a> RBRC05457 Cas-L (HEF1, NEDD9) はインテグリン下流のアダプター分子であり、その欠損はB細胞分化の異常をきたし、リンパ球のホーミング異常をきたす。またCas-Lは癌転移、進行に関与するという報告が多数なされている。Cas-Lは免疫系、癌進行、造血幹細胞研究において有用である。 東京大学医学部 (血液 ・ 腫瘍内科) 瀬尾幸子先生が作成 (2001年), 分与 (2012年)。 TT2 ES細胞を用いて作出。 Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, Jellyfish EGFP cDNA, mouse Cas-L (HEF1/NEDD9) genomic DNA Fluorescent Proteins/lacZ System Cas-L KO B6-Nedd9 KO Cas-L KO B6-Nedd9 KO Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> B6.B6CB-Nedd9<tm1Miku> B6.B6CB-Nedd9<tm1Miku> true C57BL/6コンジェニック系統。C57BL/6JJclに10世代戻し交配を行う。その後，兄妹交配にて維持。交配によりY染色体はB6置換済み。